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VOL. 2, ISSUE 2 (2026)
Influence of nitrogen levels on chlorophyll content and nitrogen uptake in paddy (Oryza sativa L.)
Authors
Trilok Nath Rai, Kedar Nath Rai, Sanjeev Kumar Rai, Rajeev Kumar Rai, Mohit Singh
Abstract
A field experiment was conducted during 2023 and 2024 to evaluate the
effect of different crop establishment methods and nitrogen levels on
chlorophyll content and nitrogen uptake of rice at various growth stages. The
study assessed chlorophyll content at 30, 60 and 90 days after sowing (DAS) and
measured nitrogen uptake at harvest. The results revealed that chlorophyll
content of rice leaves increased from 30 DAS to 60 DAS and then slightly
declined at 90 DAS during both years, indicating maximum photosynthetic
activity at the mid-growth stage. Among the crop establishment methods, M₃
consistently recorded the highest chlorophyll content at all growth stages
during both years. In 2023, chlorophyll content under M₃ reached 33.656, 40.178
and 36.078 SPAD units at 30, 60 and 90 DAS, respectively, while similar higher
values were also observed during 2024. In contrast, the lowest chlorophyll
content was generally recorded under M₁. Nitrogen levels significantly
influenced chlorophyll content, where the highest nitrogen level (N₃) resulted
in maximum chlorophyll values at all growth stages in both years, whereas the
lowest values were observed under N₁. The interaction effect also showed that
the combination of M₃N₃ produced the highest chlorophyll content across all
growth stages during both years, indicating improved physiological efficiency
and leaf greenness under this treatment combination. Nitrogen uptake by rice
was also significantly affected by crop establishment methods and nitrogen
levels. The highest nitrogen uptake was recorded under M₃ (70.26 kg ha⁻¹ in
2023 and 72.40 kg ha⁻¹ in 2024), while the lowest uptake was observed under M₁.
Similarly, nitrogen uptake increased with increasing nitrogen levels, with N₃
recording the highest uptake (78.11 kg ha⁻¹ in 2023 and 81.88 kg ha⁻¹ in 2024).
The interaction effect revealed that the combination M₃N₃ resulted in the
maximum nitrogen uptake (80.83 kg ha⁻¹ in 2023 and 86.00 kg ha⁻¹ in 2024),
whereas the lowest uptake was recorded under M₁N₁. Overall, the results
indicate that improved crop establishment methods combined with higher nitrogen
levels significantly enhanced chlorophyll content and nitrogen uptake in rice.
The combination of M₃ establishment method with N₃ nitrogen level proved most
effective in improving physiological efficiency and nutrient absorption, which
may ultimately contribute to better crop growth and productivity.
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Pages:21-27
How to cite this article:
Trilok Nath Rai, Kedar Nath Rai, Sanjeev Kumar Rai, Rajeev Kumar Rai, Mohit Singh "Influence of nitrogen levels on chlorophyll content and nitrogen uptake in paddy (<i>Oryza sativa </i>L.)". World Journal of Multidisciplinary Research and Development, Vol 2, Issue 2, 2026, Pages 21-27
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